No one species of microorganism, however, is capable of degrading all the components of given oil. Oil degrading bacteria from the bp deepwater horizon oil spill. Sp6 isolate with oil degrading activity x time hr, y axis o. Isolation and characterization of crude oil degrading. Of the diverse range of oildegrading bacteria isolated to date, less than a quarter have been obtained from marine sources. Degradation of mineral oil with a strain of acinetobacter. For examining the degradation of oil, bushnell haas medium bhm supplemented with 5g50ml of petrol, diesel and used engine oil to be used 11. Isolation and characterization of oildegrading bacteria from. A smart dress for oildegrading bacteria sciencedaily. The present study was aimed to isolate the potent cholesterol degrading bacteria from soap and vegetable oil industrial wastes and evaluate their ability for degradation of cholesterol. Fifteen crude oil degrading bacteria were isolated from oil contaminated sites in the persian gulf at khorramshahr provenance. Only crude oil degrading bacteria were capable of growing on this medium guru et al. Hydrocarbon contaminated soil and marine sources have been the common choice for the isolation of such hydrocarbon combining and degrading bacteria 2. However, knowledge on the isolation and characterization of cholesterol degrading bacteria from the industrial environment is vey scanty.
Strains from only 19 genera of eubacteria could through their obligate requirement for naclcontaining growth media be characterized as indigenous marine organisms. They characterized the abundance of colwellia and marinobacter. Inmoreextensive studies, cook and westlake determined the influence ofdegradation bysuchfactors as fertilizer and oil utilizing bacteria application to field plots in the normanwells area ofthe northwest territories and in. While hydrocarbon degrading microorganisms are ubiquitous, hydrocarbondegraders normally constitute less than 1% of the total microbial community. Characterization of crude oildegrading bacteria in a. Adeleke1,3,4 background high demand for petroleum products all over the world has led to an increase in crude oil extraction and processing. Isolation of crude oil degrading marine bacteria and assessment for biosurfactant production 2 of 7 crude oil biodegradation crude oil biodegradation experiment was performed by modifying the technique described by pirnik et al. Isolated bacterial strains were tested for hydrocarbon degradation capability. Twentyone oildegrading bacteria were isolated from bilge water. Study on the biodegradation of crude oil by free and. Dispersants set the stage for oildegrading bacteria.
Isolation and characterization of oildegrading bacteria. Pdf with the sharp increase in population and modernization of society, environmental pollution resulting from petroleum hydrocarbons has. The biochemical characteristics and 16s rrna sequence analysis of diesel degrading bacteria showed that these strains were. Isolation, identification and characterization of oil degrading bacteria isolated 433 fig. The effect of crude oil on the growth of vignamungo by pot culture experiments were analyzed the germinating and growth ability in different experimental groups showed that pseudomonas putida have more efficient than bacillus cereus.
The aim of this study was isolation and characterization of new marine oildegrading bacteria from a seawater sample. Floodgate 36 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi which were isolated. Twentythree hydrocarbondegrading bacteria strains were isolated from gas station leakingcontaminated groundwater. Analysis of bacterial diversity in two oil blocks from two.
Isolation of crude oil degrading marine bacteria and. A total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. Crude oil biodegradation in the marine environments intechopen. The community diversities of two oil reservoirs with low permeability of 1. Bacteria isolates were screened for their ability to grow in oil sludge by subculturing on mineral salts agar that was laced with 1. In recent years the microbial biodegradation of pollutants is a sustainable.
Isolation and characterization of hydrocarbondegrading bacteria. Abstract the role of plasmidborne genes in the biodegradation of chevron escravos crude oil by bacteria was determined. This strain is capable of efficient degradation of components of mineral oil alkanes, isoalkanes, and alkyl residues of the naphthene and arene fraction. Serial dilution of samples were inoculated into mineral salt medium msmbushnellhass medium supplemented with 3% nacl and adjusted to ph 7.
The identification of isolated bacteria revealed the dominance of the genus microbacterium 39. These bacteria were screened with two important factors such as growth rate on crude oil and hydrocarbon biodegradation, and then three strains were selected from 15 isolated strains for further study. In the present study, altogether 32 morphologically distinct bacteria were isolated from crude oil contaminated soil and 18 different hydrocarbon degrading bacteria were number hydrocarbon degrading bacterial strain from and. Hence, many different species are usually required for significant overall degradation. Isolation of hydrocarbon degrading bacteria from soils.
Isolation and characterization of bacteria degrading n. Parametric optimization of media for the crude oil. Atlas and bartha, 1992 was transferred to each flask and sterilized. Degradation of total petroleum hydrocarbons tphs in sand from. Species of colwellia are capable of degrading both hydrocarbons oil and dispersants, while marinobacter mainly degrade hydrocarbons. Isolation and identification of hydrocarbon degrading. Microbial biodegradation is the use of bioremediation and biotransformation methods to harness the naturally occurring ability of microbial xenobiotic metabolism to degrade, transform or accumulate environmental pollutants, including hydrocarbons e. Isolation, characterization and identification of diesel. Bioremediation for marine oil spills princeton university. Several bacteria are even known to feed exclusively on hydrocarbons 39. Microbial degradation of crude oil by fungi pregrown on. While hydrocarbondegrading microorganisms are ubiquitous, hydrocarbondegraders normally constitute less than 1% of the total microbial community. Isolation and identification of oil degrading bacteria from.
Crude oil degraders and nhexadecane degraders were enumerated adding crude oil and nhexadecane as sole. Effects of corexit ec9500a on bacteria from a beach oiled by the deepwater horizon spill. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search. One of the most important features of hydrocarbon degrading bacteria is the. Biodegradation of palm oil mill effluent pome by bacterial. Pdf study of oil degrading bacteria isolated from oil. Isolation and characterization of cholesterol degrading. Reconstructing metabolic pathways of hydrocarbondegrading. Isolation, identification and characterization of oil degrading bacteria isolated from the contaminated sites of barmer, rajasthan article pdf available september 20 with 607 reads. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 7 strains were selected from 21 isolated for. From this, 1ml of active inoculum was again transferred to fresh msm containing 1% crude oil and was used for the isolation. The modified polyelectrolytemagnetite nanocoating was applied to functionalize the cell. A new marine oildegrading consortium ctod was isolated from constanta seawater using 5% vv fuel oil, as the sole carbon source.
Bacteria are the most active agents in petroleum degradation, and they work as primary degraders of spilled oil in environment 37, 38. Most of the problems are caused due to the byproducts, which are created when then fuels are being burned. Bacterial biodegradation of crude oil using local isolates. However, knowledge on the isolation and characterization of cholesteroldegrading bacteria from the industrial environment is vey scanty. Isolation and identification of oil degrading bacteria. Isolation, identification and characterization of oil.
Two bacteria, strains ibbct6 and ibbct7 were isolated from the ctod consortium. Oct 20, 2009 oil fields occur typically at several thousand metres below ground and, at temperatures of 6090c, are considered sterile. Observations in the gulf appeared to confirm that microbes broke down a large fraction of the oil released from bps damaged well. When oil pollutants are present these hydrocarbon degrading populations increase, typically to 10% of the community alexander, 1994. Raw palm oil mill effluent pome was collected from the site of a palm oil mill industry in a sterile container and brought back to the laboratory. The crude oil degrading ability of isolated strains was analyzed.
Oil dispersants hinder oildegrading bacteria microbiology. Characterization of dioxygenases and biosurfactants. Twentythree crudeoildegrading bacteria were isolated from oilcontaminated sites near the red. There was a greater number of oil degrading bacteria near the spartina alterniflora i. The mineral salt media msm with the following composition gl. Feb 06, 20 sediments samples were collected once in a month for a period of twelve months from area surrounding ennore creek and screened for hydrocarbon degrading bacteria. Biodegradation, bacteria, diesel oil, marine environment 1. Crude oil biodegradation in the marine environments. Aerobic degradation of crude oil by microorganisms in soils from. Scientists want to use bacteria to clean up oil spills. The ability of c1, c2 and mixture of both the bacterial isolates to degrade diesel oil was monitored on the first day day zero of the study and subsequently at 5day interval for 25 days. Isolation of oil degrading bacteria from the deepwater horizon oil spill for biodiesel processing there are a number of problems with fossil fuels in our society. The oil degrading bacteria and other microorganisms degraded the chemical substance this process is termed as bioremediation. The aim of this study was isolation and characterization of new marine oil degrading bacteria from a seawater sample.
For example hassanshahian et al 2010 show that oil contamination can induce major changes in marine microbial communities at persian gulf and caspian sea, that when the pollution occur the number of crude oil degrading bacteria increased and also inhibit some catalytic enzymes. These two blocks differed significantly in salinity average 17,500 vs 40,900 mgl. Characterization of crude oildegrading bacteria in a crude. A new marine oil degrading consortium ctod was isolated from constanta seawater using 5% vv fuel oil, as the sole carbon source. Of the 1 crude oil degrading isolates 15 isolates were selected and cultivated in bh media with 1% crude oil as a sole carbon and energy source. Oil degrading bacteria are considered as the dominant hydrocarbon which helps in degrading the aquatic systems such as oceans. Isolation and characterization of vegetable oil degrading.
Microbial degradation of petroleum hydrocarbon contaminants. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search of potent hydrocarbon degrader 14. The isolated bacteria were grown on bushnell hass medium. The most common is a specific fractional distillate of petroleum fuel oil, but alternatives that are not derived from55 petroleum, such as. Of the 20 isolates, ram03, ram06, ram, and ram17 were specifically chosen based on their relatively higher growth on salt medium amended with 4 % crude oil, emulsion index, surface tension, and degradation percentage. Crude oil degrading bacteria were carried out under aerobic condition with crude oil as sole source of carbon. For the isolation of crude oil degrading bacteria, 1g of soil sample was inoculated into 100ml of mineral salt medium msm ph 6. Most reports are enforced that nalkane degrading bacteria isolated from oil contaminated sites are mesophilic strains belonging to different bacterial species such as. Isolation and characterization of vegetable oil degrading bacteria article pdf available in journal of pure and applied microbiology 52. Sediments samples were collected once in a month for a period of twelve months from area surrounding ennore creek and screened for hydrocarbon degrading bacteria. This has resulted in an increased generation of large amounts of oily waste bhattacharyya and shekdar 2003. A phylogenetic tree was constructed using the neighborjoining method in the mega 5.
Pdf petroleum hydrocarbondegrading bacteria for the. Isolation and characterization of hydrocarbondegrading. Isolation and characterisation of crude oil sludge. However, an understanding of the impacts of oil on indigenous microbial communities and identification of oil degrading microbial groups are prerequisite for directing the management and cleanup of oil contaminated ecosystems 46. The acinetobacter calcoaceticusstrain tm31 has been isolated from a microbial assemblage of a pilot plant that purifies waste water polluted with mineral oil. A common biological approach is the use of microorganisms for the degradation oil. In the present study, altogether 32 morphologically distinct bacteria were isolated from crude oil contaminated soil and 18 different hydrocarbondegrading bacteria were number hydrocarbon degrading bacterial strain from and. Hydrocarbondegrading bacteria enriched by the deepwater horizon oil spill identified by cultivation and dnasip. All marine and freshwater ecosystems contain some oildegrading bacteria. The strain bears stably inherited plasmids of sizes 120, 9, and 8 kb, which can. For several years scientists have pursued genetic modifications to enhance the ability of various types of engineered bacteria to degrade oil spills, whether on land or in sea.
Isolation and identification of bacterial consortia responsible for. Jul 22, 2016 a smart dress for oildegrading bacteria date. Bioremediation of petroleum based contaminants with biosurfactant. This can also be done by adding substances synthesized by microbes which can assist in oil recovery process 9. Obligate oildegrading marine bacteria sciencedirect. May 15, 2014 fifteen crude oil degrading bacteria were isolated from oil contaminated sites in the persian gulf at khorramshahr provenance. Isolation and identification of petroleum hydrocarbon. When oil pollutants are present these hydrocarbondegrading populations increase, typically to 10% of the community alexander, 1994. Isolation and characterisation of crude oil sludge degrading. Characterization of crude oil degrading bacteria isolated. Pure colonies obtained were stored in nutrient agar slants at 4 c for further screening and characterisation. Twentyone oil degrading bacteria were isolated from bilge water. Before the spill, waters in the gulf of mexico contained a highly diverse range of bacteria from several different phyla, or large biological families. These bacterias are capable of diverse metabolic pathways which enable them to utilize most recalcitrant petroleum hydrocarbons that are not present in fungi.
In recent years a study was undertaken to isolate crudeoil degrading bacteria from petroleumpolluted soil collected from oil fields in bahrain and kuwait and to assess their biodegradation potential. Identification and characterisation of oil sludge degrading bacteria. Isolation and characterization of new marine oildegrading. Dispersants set the stage for oil degrading bacteria.
The current study aims to isolate oil degrading bacteria from soil and to test the degrading ability of the isolated bacteria on different types of oils. Microbial products for eor oil degrading microbes can be isolated from oil contaminated soil or water waste. The isolated bacteria were used as inoculum and added in 250ml flask containing 100ml bushnell hass broth and 1% crude oil, only four organisms showed potency to degrade. Dispersant and dispersant oil treated samples increased the abundance of colwellia from 1% to 2643. The overall degradation ofthe crude oil cannot be deducedfromthedatareported. Parametric optimization of media for the crude oil degrading. We have measured rates of biodegradation how fast oil was degraded in deep waters and sediments in the northern gulf of mexico. About 50ml medium was dispensed in 250ml conical flasks. Thus, if there was an oil spill today, we could better predict where oil would go, since the model could include our measurements of the speed of. Isolation and characterization of oildegrading bacteria from bilge water seed culture broth were transferred into a 500 ml erlenmeyer flask containing 250 ml of onr7a medium supplemented with 1% wv of sterile arabian light crude oil. The group collected samples from the gulf of mexico and treated them with oil, dispersant, or a combination of the two. These bacterial cultures had 16s rrna gene sequences. Only two nacldependent hydrocarbondegrading strains of the firmicutes and. This bacterium is of particular interest as it is a cosmopolitan marine bacterium that blooms in oil.
Determination of predominant species of oildegrading. The sample was transported to the laboratory in an ice box and analyzed for microbiological and physicochemical properties. The burning of fossil fuels creates energy that runs the entire economy of our planet. Sep 21, 2016 a total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. For the treatment of waste and waste water the use of the oil degrading bacteria has been strongly applied in controlled systems hazen, et, al, 2010. The oil degrading bacteria are mostly common in sea water all over the world and are particularly essential to address oil polluted waters as soon as possible as the contamination can have the potential to damage fishery resources and affect the health of those animals and humans that consume contaminated fish.
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